The Canadian Society of Microbiologists
Advancing Microbiology, Connecting Microbiologists
The Canadian Society of Microbiologists (CSM) seeks to advance microbiology in all its aspects and to facilitate the interchange of ideas between microbiologists.
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CSM Murray Award 2022 Lecture Dr. Roger C. Levesque, Université Laval, Laval, Québec
Title: The crisis in antibiotic resistance and in bacterial virulence: Pseudomonas aeruginosa as a model system
Summary: The year 2020 marked the 20th anniversary of the publication of the Pseudomonas aeruginosa PAO1 whole genome sequence (WGS). The PAO1 WGS coupled to bacterial genetics has been a driver for studies on virulence, antimicrobial resistance (AMR) and major microbial OMICS advances. We used P. aeruginosa to expand an animal model of chronic lung infection, and to road-test strategies for high throughput screening to identify genes for in vivo maintenance. We developed PCR-based signature-tagged mutagenesis (STM-PCR) and in vivo expression technology (IVET) pointing to key genes for in vivo virulence.